squalene

what a day...seyes bercelaru...dgn tu nie..
hurmm...
people seems to have problem wuf me...
apa silap aku ar...

aku agak bercelaru dgn dgn my result...
ok...

i found one of the metabolite that associate with mevalonate - squalene...quite high jugaklah dia punya abundances..but the problem is..i couldnt detect it bila aku tukar derivatization protocol aku..hmm...

i'm guessing the metabolite has a very fast turn over rate...kot..sebab derivatization using microwave kejap jer..3 minit pastu trus run...

bila aku change to shaking memang tak detectec....pulak tu kat condition 30C jer...
aku tak tunjukpun result tu kat my supervisor...

my objective is profiling...benda tu adalah objective diorang dulu2...kalu aku tunjuk sekarang cam aku nak cari famelah pulak...lagipun results to tak der reproducibility...tapi kalu aku run lagi samples aku ...nescaya akan kuarkan metabolite tu...huhuh...kang terpkasalak aku tukar objective aku...tak mo....pening2...
i asked kwn aku..and instead of giving opinion...dia cakap...ko tunjuk hebat kaa???...ahaha terkesima jap...btulgak...
jadi biarkan dulu..ahaha...

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GC GC TOF still tak run lagi...budak MPOB tu still guna until next week kot..adeh...jadi aku ingat mau run samples aku weekend untuk GC...
tadipun aku run sample on D4 Alanine...couldnt detect it...bukan tak detect tapi aku malas nak cari..ahaha...but setelah run tadi tengok..aiyo..aku kena modified balik run time aku@ solvent delay...from 6.2 to about 5....sebab dia kuat kat 5.87...ahah..aku berjaya gunakan pencarian SIR dgn jayanyer...ahaha....


hoping everything goes will for me weekend nie InsyaAllah...sebab aku dah takleh tunggu2 dah...aku target end of this year aku settlekan TMS ---GCMS dan GC x GC TOF...

bab MVA analysis tak masuk lagi...tu lagi pening...aku ada bukak account kat this online database under institute aku..tapi aku tak cakappun kat my supervisor...huhuh...sharing is caring tapi...most of scientist laa nie...ader ke nak gonsi abisan??? hmm...i wonder...ahaha...

ok dah enough wuf lab stuf...balik umah berehat...ahaha..k nak layan AFV kat youtube lagi best ahahah